Hi Pankaj,
Yes it is possible to do so.
First you will need to import the images into medInria. Basically what you have to do is, from the Browser area, navigate to the folder where your images are and then right click -> import.
Then, in order to do tractography, please refer to the full instructions on this page:
med.inria.fr/docs/23-doc-diffusion-conf .
Let us know if it doesn't work!